Brain Images

There is a quote on this:
"if you can see it, you can be it"

Billie Jean King (1943 -)

Here are images all from my previous and current projects.

Note: many of them are not published.

Brain slide scannings
(Immunofluorescence)

A brief explanation on these images acquired by me previously:

Figure 1 (unpublished): overexpression of a protein (we identifed from dopamingergic neuron profiling), in a TH-Cre driver mouse line (unpublished).
Figure 2 (unpublished): enlarged view in Figure 1.
Figure 3: examing dorsomedial nucleus accumbens (dmNAc) Tac2-expressing cells and their projection.
Figure 4 (unpublished): injecting a Cre-dependent Gq virus into dmNAc in Tac2-Cre mice.
Figure 5 (unpublished): Th+ cells in the midbrain, in a genetic mutant mice (related to diabetes project).
Figure 6: expression pattern of the dmPAG Tac2+ cells.
Figure 7 (unpublished). Using TMEM119-EGFP mice to examine how short-term HFD affects microglia morphology (brain slide). Image acquired together with my student Emily (summer student working with me).
Figure 8 (unpublished). Confocal image from a TMEM119-EGFP mouse fed with short-term HFD. We aimed to examine microglia morphology in a higher maganification. Image acquired together with my student Emily (summer student working with me).

Research showcase_AAV + TH-Cre mouse

AAV + TH-Cre mouse

NAc_tac2+ cells

Gq expression in dmNAc Tac2+ cells

Examing Th+ signals in midbrain in a genetic mutant mouse

PAG Tac2 neuron_03

TMEM119-EGFP mice treated with short-term HFD (3d)

Microglia morphology analysis_TMEM119-EGFP mice

Phagocytosis, Abeta Uptake
(Images or live-cell imaging)

Examing phagocytosis in astrocytes or microglia. To establish this important functional readout, I have to use multiple ways to perform this experiment. These images are also acquired by me (unless further noted)

Figure 1: iGIRKO/5xFAD mice, examing Abeta uptake using IHC (GFAP and Abeta).
Figure 2 (demo experiment): in 3xTg mice, examing microglia phagocytosis (IBA1 and Abeta). Image acquired by me and my proud students in Bordeaux School of Neuroscience (07/2024, Bordeaux, France)
Figure 3 (unpublished): live cell imaging, examined by Incucyte S3. SIIM-A9 cells uptaking Abeta555.
Figure 4 (unpublished): whole-brain CLARITY, examing blood vessel (lectin) and Abeta deposition, in 5xFAD mouse brain.
Figute 5 (demo experiment): in BV2 cells, examing cellular uptake of Abeta555. Image acquired by me and my proud students in Bordeaux School of Neuroscience (07/2024, Bordeaux, France)
Figure 6 (unpublished): examing gliosis (or reactive astrocyte) in 5xFAD brain (with or without insulin receptor KO)
Figure 7 and Figure 8 (unpublished): SIM-A9 cells uptaking E.coli(488). Cells are fixed but not stained.

Astrocyte IRKO + Amyloid-beta

Microglia phagocytosis

Incucyte live cell imaging (SIM-A9 microglial cells uptake Abeta555)

segital view of cleared 5xFAD brain

Live cell imaging_BV2 cells uptaking Abeta555

Reactive astrocytes_GFAP staining in 5xFAD mouse brain

SIM-A9 phagocytosis of E.coli (488)_2

SIM-A9 phagocytosis of E.coli (488)

Abeta deposition along blood vessels in the brain, is also one of my interested areas. Using whole-brain CLARITY, I am studying how astrocyte insulin receptor (IR) KO can affect vascular amyloid deposition.

Left: IR flos control mice.
Right: 5xFAD or iGIRKO/5xFAD mouse brain (sorry I will not tell you what it is until this is published)

IRloxP ctr_Composite

5xFAD_Composite

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Brain images

Brain Images

Brain slide scannings (Immunofluorescence)

Phagocytosis, Abeta Uptake (Images or live-cell imaging)

Brain slide scannings
(Immunofluorescence)

Phagocytosis, Abeta Uptake
(Images or live-cell imaging)